Bioprinted aligned muscle strip, WWD example package

What it describes

A printed strip whose authoritative geometry and print parameters live in an embedded 3MF part. The 3MF part is manifested with role opaque.

Geometry is delegated. The component points at process/print-job.3mf and marks it authoritative.
A rewriter that does not understand an asset with role opaque preserves its bytes.
Nozzle temperature is a control field whose imposed_by points into the 3MF part.
The acceptance criteria are the tissue criteria, not print criteria.

Package facts

Every entry, with its manifest role.

ZIP entries
Entry	Bytes	Role	Media type
mimetype	23	(package identity)	text/plain
wwd/manifest.json	728	manifest	application/json
wwd/design.json	8079	design	application/vnd.wwd.design+json
process/print-job.3mf	756	opaque	application/vnd.ms-package.3dmanufacturing-3dmodel+xml

Identity, frame, dimensions and biology.

Bioprinted aligned muscle strip
Identifier	component/bioprinted-strip
Kind	contractile_tissue
Dimensions	length 12 mm, width 2 mm, thickness 0.3 mm
Frame	right-handed, axes x y z, units mm, origin centroid of loop-a bore
Validity window	hours_post_seeding 168 to 336
Geometry	process/print-job.3mf, format 3mf, authoritative true

Populations
Role	Cell type	Species	Derivation
primary	skeletal muscle myoblast CL:0000515	Homo sapiens NCBITaxon:9606	primary

Fields
Property	Causal role	Provenance	Value
wwd:ecm_stiffness	control	designed	12 kPa · imposed_by protocol/gel-casting#step-2
wwd:matrix_formulation	control	designed	composition: collagen_i 0.6, fibrin 0.4 · imposed_by protocol/gel-casting#step-1
wwd:seeding_density_volumetric	control	designed	2.0e7 cells/mL · imposed_by protocol/seeding#step-1
wwd:stimulation_frequency	control	designed	1 Hz · imposed_by protocol/maturation#e-stim
wwd:fiber_alignment	response	designed	0.72 1
wwd:peak_twitch_force	response	designed	550 uN
wwd:print_nozzle_temperature	control	designed	22 degC · imposed_by process/print-job.3mf#extruder-1

Interfaces
Identifier	Modality	Role
iface/loop-a	mechanical	attachment_loop
iface/loop-b	mechanical	attachment_loop

Measurand, statistic, comparator, limit and method, as written in the design document.

Criteria
Tag	Title	Measurand	Statistic	Limit	Criticality	Axis	Method	n
AC-001	Median twitch force	peak_twitch_force	median	>= 500 uN	critical	response	WWD-TWITCH-001	12
AC-002	Twitch force consistency	peak_twitch_force	cv	<= 0.2 1	major	response	WWD-TWITCH-001	not stated
AC-003	Alignment floor	fiber_alignment	median	>= 0.65 1	major	response	WWD-ALIGN-001	not stated
PC-001	Gel stiffness compliance	ecm_stiffness	value	range 11.0 to 13.0 kPa	major	control	WWD-RHEO-001	not stated