Example package
Stiffness-graded muscle strip
A strip whose matrix stiffness varies over the geometry. The stiffness field is backed by node values in a delegated VTU part carried inside the package.
What it describes
A strip whose matrix stiffness varies over the geometry. The stiffness field is backed by node values in a delegated VTU part carried inside the package.
Readings
- A property slot holds either a quantity string or a field object. Here matrix stiffness is a field object with backing mesh_attribute.
- The field names its store, its association, its unit and its interpolation. The numbers live in fields/stiffness.vtu.
- The property term, the causal role and the provenance are the same as in the uniform package.
Archive entries
Every entry, with its manifest role.
| Entry | Bytes | Role | Media type |
|---|---|---|---|
| mimetype | 23 | (package identity) | text/plain |
| wwd/manifest.json | 714 | manifest | application/json |
| wwd/design.json | 7664 | design | application/vnd.wwd.design+json |
| fields/stiffness.vtu | 865 | opaque | application/vnd.vtk.unstructured-grid+xml |
Component
Identity, frame, dimensions and biology.
| Identifier | component/graded-strip |
|---|---|
| Kind | contractile_tissue |
| Dimensions | length 12 mm, width 2 mm, thickness 0.3 mm |
| Frame | right-handed, axes x y z, units mm, origin centroid of loop-a bore |
| Validity window | hours_post_seeding 168 to 336 |
| Geometry | not carried in this package |
| Role | Cell type | Species | Derivation |
|---|---|---|---|
| primary | skeletal muscle myoblast CL:0000515 | Homo sapiens NCBITaxon:9606 | primary |
| Property | Causal role | Provenance | Value |
|---|---|---|---|
| wwd:ecm_stiffness | control | designed | field, backing mesh_attribute, store fields/stiffness.vtu, association node, unit kPa · imposed_by protocol/gel-casting#step-2 |
| wwd:matrix_formulation | control | designed | composition: collagen_i 0.6, fibrin 0.4 · imposed_by protocol/gel-casting#step-1 |
| wwd:seeding_density_volumetric | control | designed | 2.0e7 cells/mL · imposed_by protocol/seeding#step-1 |
| wwd:stimulation_frequency | control | designed | 1 Hz · imposed_by protocol/maturation#e-stim |
| wwd:fiber_alignment | response | designed | 0.72 1 |
| wwd:peak_twitch_force | response | designed | 550 uN |
| Identifier | Modality | Role |
|---|---|---|
| iface/loop-a | mechanical | attachment_loop |
| iface/loop-b | mechanical | attachment_loop |
| Identifier | Kind |
|---|---|
| region/active | whole_component |
| region/loop-a | geometry_subset |
| region/loop-b | geometry_subset |
Acceptance criteria
Measurand, statistic, comparator, limit and method, as written in the design document.
| Tag | Title | Measurand | Statistic | Limit | Criticality | Axis | Method | n |
|---|---|---|---|---|---|---|---|---|
| AC-001 | Median twitch force | peak_twitch_force | median | >= 500 uN | critical | response | WWD-TWITCH-001 | 12 |
| AC-002 | Twitch force consistency | peak_twitch_force | cv | <= 0.2 1 | major | response | WWD-TWITCH-001 | not stated |
| AC-003 | Alignment floor | fiber_alignment | median | >= 0.65 1 | major | response | WWD-ALIGN-001 | not stated |
| PC-001 | Gel stiffness compliance | ecm_stiffness | value | range 11.0 to 13.0 kPa | major | control | WWD-RHEO-001 | not stated |
- The other example packages.
- The Core registries: every property term, assay and closed vocabulary.
- The .wwd file format reference.