Aligned contractile muscle strip, as built, WWD example package

What it describes

The same strip after a lot was made and tested. The package adds a results document, an assay evidence CSV, and a provenance graph.

Each measured result names one criterion tag. The criteria themselves are unchanged from the as-designed package.
A yield statement records 12 units tested and 12 meeting AC-001.
Context is recorded separately from control and response. Passage number is carried as context.
The provenance graph holds 8 entities and 12 edges across expansion, fabrication and assay activities.

Package facts

Every entry, with its manifest role.

ZIP entries
Entry	Bytes	Role	Media type
mimetype	23	(package identity)	text/plain
wwd/manifest.json	1065	manifest	application/json
wwd/design.json	7230	design	application/vnd.wwd.design+json
wwd/results.json	5046	measured_result_data	application/vnd.wwd.results+json
data/twitch.csv	92	assay_evidence	text/csv

Identity, frame, dimensions and biology.

Aligned contractile muscle strip
Identifier	component/muscle-strip
Kind	contractile_tissue
Dimensions	length 12 mm, width 2 mm, thickness 0.3 mm
Frame	right-handed, axes x y z, units mm, origin centroid of loop-a bore
Validity window	hours_post_seeding 168 to 336
Geometry	not carried in this package

Populations
Role	Cell type	Species	Derivation
primary	skeletal muscle myoblast CL:0000515	Homo sapiens NCBITaxon:9606	primary

Fields
Property	Causal role	Provenance	Value
wwd:ecm_stiffness	control	designed	12 kPa · imposed_by protocol/gel-casting#step-2
wwd:matrix_formulation	control	designed	composition: collagen_i 0.6, fibrin 0.4 · imposed_by protocol/gel-casting#step-1
wwd:seeding_density_volumetric	control	designed	2.0e7 cells/mL · imposed_by protocol/seeding#step-1
wwd:stimulation_frequency	control	designed	1 Hz · imposed_by protocol/maturation#e-stim
wwd:fiber_alignment	response	designed	0.72 1
wwd:peak_twitch_force	response	designed	550 uN

Interfaces
Identifier	Modality	Role
iface/loop-a	mechanical	attachment_loop
iface/loop-b	mechanical	attachment_loop

Measurand, statistic, comparator, limit and method, as written in the design document.

Criteria
Tag	Title	Measurand	Statistic	Limit	Criticality	Axis	Method	n
AC-001	Median twitch force	peak_twitch_force	median	>= 500 uN	critical	response	WWD-TWITCH-001	12
AC-002	Twitch force consistency	peak_twitch_force	cv	<= 0.2 1	major	response	WWD-TWITCH-001	not stated
AC-003	Alignment floor	fiber_alignment	median	>= 0.65 1	major	response	WWD-ALIGN-001	not stated
PC-001	Gel stiffness compliance	ecm_stiffness	value	range 11.0 to 13.0 kPa	major	control	WWD-RHEO-001	not stated

Realization, measured results, yield and lineage.

Realization
Identifier scheme	local
Design revision tested	1.3.0
Yield	12 of 12 units meeting AC-001
Context recorded	wwd:passage_number = 6 1
Provenance graph	8 entities, 12 edges
Activity kinds	assay, expansion, fabrication

Measured results
Criterion	Status	Value	Statistic	n observed	Provenance	Causal role
AC-001	pass	612 uN	median	12	measured	response
AC-002	pass	0.14 1	cv	12	measured	response
AC-003	pass	0.71 1	median	5	measured	response
PC-001	pass	12.4 kPa	value	not stated	measured	control

A consumer recomputes every verdict from the limit, the statistic and the observed value. The recorded status is not trusted.