Example package
Muscle strip at 20 kPa matrix stiffness
The same component and the same criteria, built at a higher matrix stiffness. Matrix stiffness moves from 12 kPa to 20 kPa and the process criterion range moves with it.
What it describes
The same component and the same criteria, built at a higher matrix stiffness. Matrix stiffness moves from 12 kPa to 20 kPa and the process criterion range moves with it.
Readings
- One control value changes. The response criteria AC-001 to AC-003 are identical to the 12 kPa package.
- The measured results differ: median twitch force 703 uN against 612 uN, median alignment 0.79 against 0.71.
- The process criterion PC-001 tracks the control value, from 11.0 to 13.0 kPa to 19.0 to 21.0 kPa.
Archive entries
Every entry, with its manifest role.
| Entry | Bytes | Role | Media type |
|---|---|---|---|
| mimetype | 23 | (package identity) | text/plain |
| wwd/manifest.json | 864 | manifest | application/json |
| wwd/design.json | 7230 | design | application/vnd.wwd.design+json |
| wwd/results.json | 5046 | measured_result_data | application/vnd.wwd.results+json |
Component
Identity, frame, dimensions and biology.
| Identifier | component/muscle-strip |
|---|---|
| Kind | contractile_tissue |
| Dimensions | length 12 mm, width 2 mm, thickness 0.3 mm |
| Frame | right-handed, axes x y z, units mm, origin centroid of loop-a bore |
| Validity window | hours_post_seeding 168 to 336 |
| Geometry | not carried in this package |
| Role | Cell type | Species | Derivation |
|---|---|---|---|
| primary | skeletal muscle myoblast CL:0000515 | Homo sapiens NCBITaxon:9606 | primary |
| Property | Causal role | Provenance | Value |
|---|---|---|---|
| wwd:ecm_stiffness | control | designed | 20 kPa · imposed_by protocol/gel-casting#step-2 |
| wwd:matrix_formulation | control | designed | composition: collagen_i 0.6, fibrin 0.4 · imposed_by protocol/gel-casting#step-1 |
| wwd:seeding_density_volumetric | control | designed | 2.0e7 cells/mL · imposed_by protocol/seeding#step-1 |
| wwd:stimulation_frequency | control | designed | 1 Hz · imposed_by protocol/maturation#e-stim |
| wwd:fiber_alignment | response | designed | 0.72 1 |
| wwd:peak_twitch_force | response | designed | 550 uN |
| Identifier | Modality | Role |
|---|---|---|
| iface/loop-a | mechanical | attachment_loop |
| iface/loop-b | mechanical | attachment_loop |
| Identifier | Kind |
|---|---|
| region/active | whole_component |
| region/loop-a | geometry_subset |
| region/loop-b | geometry_subset |
Acceptance criteria
Measurand, statistic, comparator, limit and method, as written in the design document.
| Tag | Title | Measurand | Statistic | Limit | Criticality | Axis | Method | n |
|---|---|---|---|---|---|---|---|---|
| AC-001 | Median twitch force | peak_twitch_force | median | >= 500 uN | critical | response | WWD-TWITCH-001 | 12 |
| AC-002 | Twitch force consistency | peak_twitch_force | cv | <= 0.2 1 | major | response | WWD-TWITCH-001 | not stated |
| AC-003 | Alignment floor | fiber_alignment | median | >= 0.65 1 | major | response | WWD-ALIGN-001 | not stated |
| PC-001 | Gel stiffness compliance | ecm_stiffness | value | range 19.0 to 21.0 kPa | major | control | WWD-RHEO-001 | not stated |
Results document
Realization, measured results, yield and lineage.
| Identifier scheme | local |
|---|---|
| Design revision tested | 1.3.0 |
| Yield | 12 of 12 units meeting AC-001 |
| Context recorded | wwd:passage_number = 6 1 |
| Provenance graph | 8 entities, 12 edges |
| Activity kinds | assay, expansion, fabrication |
| Criterion | Status | Value | Statistic | n observed | Provenance | Causal role |
|---|---|---|---|---|---|---|
| AC-001 | pass | 703 uN | median | 12 | measured | response |
| AC-002 | pass | 0.11 1 | cv | 12 | measured | response |
| AC-003 | pass | 0.79 1 | median | 5 | measured | response |
| PC-001 | pass | 20.3 kPa | value | not stated | measured | control |
A consumer recomputes every verdict from the limit, the statistic and the observed value. The recorded status is not trusted.
- The other example packages.
- The Core registries: every property term, assay and closed vocabulary.
- The .wwd file format reference.