NeuronTile, MEA-ready cortical culture, WWD example package

What it describes

A 24 by 24 mm neural culture on a microelectrode array, built and tested. Two iPSC-derived populations, three interfaces, five criteria.

The same field, criterion and result machinery applies to a component that is not printed and has no bulk geometry.
Two populations are declared with distinct biological roles, both from Homo sapiens.
Electrical recording, electrical stimulation and optical imaging access are separate interfaces on one component.
The yield statement records 8 units tested and 7 meeting NT-001.
The provenance graph holds 22 entities and 41 edges, including split and pooling activities.

Package facts

Every entry, with its manifest role.

ZIP entries
Entry	Bytes	Role	Media type
mimetype	23	(package identity)	text/plain
wwd/manifest.json	864	manifest	application/json
wwd/design.json	8595	design	application/vnd.wwd.design+json
wwd/results.json	9659	measured_result_data	application/vnd.wwd.results+json

Identity, frame, dimensions and biology.

NeuronTile, MEA-ready cortical culture
Identifier	component/neuron-tile
Kind	neural_culture
Dimensions	length 24 mm, width 24 mm, thickness 0.15 mm
Frame	right-handed, axes x y z, units mm, origin substrate center
Validity window	days_in_vitro 21 to 42
Geometry	not carried in this package

Populations
Role	Cell type	Species	Derivation
primary	glutamatergic neuron CL:0000679	Homo sapiens NCBITaxon:9606	ipsc_derived
support	astrocyte CL:0000127	Homo sapiens NCBITaxon:9606	ipsc_derived

Fields
Property	Causal role	Provenance	Value
wwd:seeding_density_areal	control	designed	1500 cells/mm2 · imposed_by protocol/plating#step-3
wwd:medium_formulation	control	designed	1 1 · imposed_by protocol/feeding#schedule
wwd:active_electrode_fraction	response	designed	0.80 1
wwd:mean_firing_rate	response	designed	2.5 Hz
wwd:network_burst_rate	response	designed	0.15 Hz

Regions
Identifier	Kind
region/active-culture	whole_component
region/electrode-array	geometry_subset

Measurand, statistic, comparator, limit and method, as written in the design document.

Criteria
Tag	Title	Measurand	Statistic	Limit	Criticality	Axis	Method	n
NT-001	Active electrode fraction	active_electrode_fraction	proportion	>= 0.8 1	critical	response	WWD-MEA-001	not stated
NT-002	Mean firing rate floor	mean_firing_rate	median	>= 0.1 Hz	critical	response	WWD-MEA-001	not stated
NT-003	Network burst rate	network_burst_rate	median	>= 0.0167 Hz	critical	response	WWD-BURST-001	not stated
NT-004	Network burst participation	electrodes_in_network_burst	proportion	>= 0.25 1	critical	response	WWD-BURST-001	not stated
NT-006	Culture age at ship	days_in_vitro	value	range 28 to 35 d	critical	control	WWD-DIV-001	not stated

Realization, measured results, yield and lineage.

Realization
Identifier scheme	local
Design revision tested	0.4.0
Yield	7 of 8 units meeting NT-001
Context recorded	wwd:source_line = CVCL_1E52, wwd:days_in_vitro = 28 1
Provenance graph	22 entities, 41 edges
Activity kinds	differentiation, expansion, pooling, split

Measured results
Criterion	Status	Value	Statistic	n observed	Provenance	Causal role
NT-001	pass	0.87 1	proportion	60	measured	response
NT-002	pass	3.1 Hz	median	52	measured	response
NT-003	pass	0.11 Hz	median	not stated	measured	response
NT-004	pass	0.62 1	proportion	60	measured	response
NT-006	pass	30 d	value	not stated	measured	control

A consumer recomputes every verdict from the limit, the statistic and the observed value. The recorded status is not trusted.