Muscle strip with a simulation envelope, WWD example package

What it describes

The as-built strip with a finite-element model, one solver run and two simulated results added to the results document.

A run declares completion and convergence as two separate facts, and names the solver and its version.
Simulated results carry provenance simulated. Measured results carry provenance measured. They are separate lists.
The simulated peak twitch force of 648 uN answers no acceptance criterion. AC-001 is satisfied by the measured 612 uN.

Package facts

Every entry, with its manifest role.

ZIP entries
Entry	Bytes	Role	Media type
mimetype	23	(package identity)	text/plain
wwd/manifest.json	864	manifest	application/json
wwd/design.json	7230	design	application/vnd.wwd.design+json
wwd/results.json	8498	measured_result_data	application/vnd.wwd.results+json

Identity, frame, dimensions and biology.

Aligned contractile muscle strip
Identifier	component/muscle-strip
Kind	contractile_tissue
Dimensions	length 12 mm, width 2 mm, thickness 0.3 mm
Frame	right-handed, axes x y z, units mm, origin centroid of loop-a bore
Validity window	hours_post_seeding 168 to 336
Geometry	not carried in this package

Populations
Role	Cell type	Species	Derivation
primary	skeletal muscle myoblast CL:0000515	Homo sapiens NCBITaxon:9606	primary

Fields
Property	Causal role	Provenance	Value
wwd:ecm_stiffness	control	designed	12 kPa · imposed_by protocol/gel-casting#step-2
wwd:matrix_formulation	control	designed	composition: collagen_i 0.6, fibrin 0.4 · imposed_by protocol/gel-casting#step-1
wwd:seeding_density_volumetric	control	designed	2.0e7 cells/mL · imposed_by protocol/seeding#step-1
wwd:stimulation_frequency	control	designed	1 Hz · imposed_by protocol/maturation#e-stim
wwd:fiber_alignment	response	designed	0.72 1
wwd:peak_twitch_force	response	designed	550 uN

Interfaces
Identifier	Modality	Role
iface/loop-a	mechanical	attachment_loop
iface/loop-b	mechanical	attachment_loop

Measurand, statistic, comparator, limit and method, as written in the design document.

Criteria
Tag	Title	Measurand	Statistic	Limit	Criticality	Axis	Method	n
AC-001	Median twitch force	peak_twitch_force	median	>= 500 uN	critical	response	WWD-TWITCH-001	12
AC-002	Twitch force consistency	peak_twitch_force	cv	<= 0.2 1	major	response	WWD-TWITCH-001	not stated
AC-003	Alignment floor	fiber_alignment	median	>= 0.65 1	major	response	WWD-ALIGN-001	not stated
PC-001	Gel stiffness compliance	ecm_stiffness	value	range 11.0 to 13.0 kPa	major	control	WWD-RHEO-001	not stated

Realization, measured results, yield and lineage.

Realization
Identifier scheme	local
Design revision tested	1.3.0
Yield	12 of 12 units meeting AC-001
Context recorded	wwd:passage_number = 6 1
Provenance graph	8 entities, 12 edges
Activity kinds	assay, expansion, fabrication

Measured results
Criterion	Status	Value	Statistic	n observed	Provenance	Causal role
AC-001	pass	612 uN	median	12	measured	response
AC-002	pass	0.14 1	cv	12	measured	response
AC-003	pass	0.71 1	median	5	measured	response
PC-001	pass	12.4 kPa	value	not stated	measured	control

A consumer recomputes every verdict from the limit, the statistic and the observed value. The recorded status is not trusted.

Simulation models and runs
Identifier	Kind or solver	Detail
model/fea-contraction	continuum_mechanics	predict peak twitch force from geometry and alignment. Laws active_hill, passive_neohookean. Symmetry transverse_isotropic.
run/fea-001	FEBio 4.4.2	completion yes, convergence yes

Simulation results
Identifier	Quantity	Value	Provenance	Causal role
sr-0001	peak_twitch_force	648 uN	simulated	response
sr-0002	unloaded_shortening	0.081 1	simulated	response